RESUMO Objetivo: Este estudo teve por objetivo determinar a expressão de esclerostina (SOST) em pacientes com fraturas vertebrais osteoporóticas por compressão na coluna toracolombar antes e depois da Cifoplastia Percutânea (PKP). Métodos: Os níveis de SOST no soro foram quantificados por meio de um Ensaio de Imunoadsorção Ligado à Enzima (ELISA) sanduíche, realizado no pré-operatório e aos seis meses pós-operatório. Foram também registrados a altura do corpo vertebral anterior, os ângulos cifóticos, e os valores obtidos na escala analógica visual (VAS). Resultados: O nível de SOST no soro foi altamente expresso nos pacientes, e permaneceu correlacionado negativamente com a densidade mineral óssea (DMO). As alturas vertebrais, os ângulos cifóticos locais, e as pontuações obtidas na escala VAS foram significativamente melhores após o PKP. No entanto, o SOST foi correlacionado positivamente com a DMO aos seis meses pós-operatório. Conclusão: O PKP foi uma estratégia eficaz para o tratamento de fraturas vertebrais osteoporóticas por compressão na coluna toracolombar, melhorando os níveis de DMO e diminuindo os níveis de SOST no soro. Nível de Evidência II, Estudo prospectivo comparativo.

ABSTRACT Objective: This study sought to determine serum sclerostin (SOST) expression in patients with osteoporotic thoracolumbar vertebral compression fractures before and after percutaneous kyphoplasty (PKP). Methods: Serum SOST levels were quantified with a sandwich enzyme-linked immunosorbent assay (ELISA) preoperatively and six months postoperatively. Anterior vertebral height, kyphotic angles, and Visual Analogue Scale (VAS) scores were also recorded. Results: Serum SOST was highly expressed in patients and remained negatively correlated with bone mineral density (BMD). Vertebral heights, local kyphotic angles, and VAS scores were all significantly improved after PKP. However, serum SOST was positively correlated with BMD six months after surgery. Conclusion: PKP was an effective treatment strategy for osteoporotic thoracolumbar vertebral compression fractures, improving BMD and decreasing serum SOST levels. Level of Evidence II, Prospective comparative study.

Abstract In this work, the effects of 1-methylcyclopropene (1-MCP) on texture properties, titratable acidity (TA) and total soluble solids (TSS) in blueberry were examined during long term storage and simulated transportation. Blueberry fruits were stored at 0 ± 0.5 °C after being treated with 0.1, 0.2 and 0.3μL L-11-MCP. After 40 and 80 days of storage, quickly put 8 punnets into a foam box with commercial cold pack to simulate the commercial transportation for 2 days at 25 °C. There were considerable differences in the texture properties during long term storage and simulated transportation with 1-MCP. The 0.3 μL L-1 1-MCP can effectively decreased the decline of gumminess and chewiness, retarded the reduction of TA, and delayed blueberry fruit softening. There was significant positive correlation among firmness, springiness, cohesiveness, gumminess, chewiness, resilience, TSS, and TA. These results demonstrate that the 0.3 μL L-1 1-MCP combines with commercial polyethylene bags were successful effect in maintaining the texture properties of blueberry.